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mouse anti atf4  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse anti atf4
    Mouse Anti Atf4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 951 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+atf4/ATF-4+Antibody/pm41650935-395-88-89
    Average 96 stars, based on 951 article reviews
    mouse anti atf4 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    SDS Page:

    Article Title: MYC Drives mRNA Pseudouridylation to Mitigate Proliferation-Induced Cellular Stress during Cancer Development
    Article Snippet: Cell lysates were prepared using standard SDS sample buffer, and protein concentrations were determined using a Bio-Rad Protein Assay Kit II. .. Proteins (20−50 μg) were separated on SDS-PAGE and transferred to nitrocellulose membranes, which were then probed with the following primary antibodies: rabbit anti-ASNS (1:1,000, Proteintech, 14681-1-AP, RRID: AB_2060119), mouse anti-ATF4 (1:400, Santa Cruz Biotechnology, sc390063, RRID: AB_2810998), rabbit anti-DENR (1:2,000, Proteintech, 10656-1-AP, RRID: AB_2092124), rabbit anti-DDIT3/CHOP (L63F6, 1:500, Cell Signaling Technology, 2895, RRID: AB_2089254), rabbit anti-GAPDH (FL335, 1:1,000, Santa Cruz Biotechnology, sc25778, RRID: AB_10167668), rabbit anti-GCN2 (1:2,000, Thermo Fisher Scientific, 720463, RRID: AB_2762413), rabbit anti-GCN2 phospho-T889 (1:1,000, Abcam, ab75836, RRID: AB_1310260), rabbit anti-MCTS1 (1:1,000, Thermo Fisher Scientific, PA5-31020, RRID: AB_2548494), rabbit anti-MYC (c-MYC, N262, 1:1,000, Santa Cruz Biotechnology, sc764, RRID: AB_631276), mouse anti-Myc tag (clone 4A6, 1:1,000, Millipore, 05724, RRID: AB_309938), mouse anti-MYCN (B8.4.B, 1:400, Santa Cruz Biotechnology, sc53993, RRID: AB_831602), rabbit anti-PHGDH (1:300, Sigma-Aldrich, HPA021241, RRID: AB_1855299), rabbit anti-PSAT1 (1:3,000, Novus Biologicals, 21020002, RRID: AB_2172599), rabbit anti-PUS7 (1:1,000, Thermo Fisher Scientific, PA5-54983, RRID: AB_2646152), rabbit anti-SLC7A5 (LAT1, 1:1,000, Cell Signaling Technology, 5347, RRID: AB_10695104), rabbit anti-SLC7A11/xCT (D2M7A, 1:1,000, Cell Signaling Technology, 12691, RRID: AB_2687474), rabbit anti-β-actin (1:2,000, Thermo Fisher Scientific, MA5-15739, RRID: AB_10979409), and mouse anti-α-tubulin (B-5-1-2, 1:5,000, Sigma-Aldrich, T5168, RRID: AB_477579). .. Horseradish peroxidase–conjugated goat anti-mouse (Jackson ImmunoResearch, 115-035-146, RRID: AB_2307392), and goat anti-rabbit IgG (Jackson ImmunoResearch, 111-035-046, RRID: AB_2337939) were used as secondary antibodies.

    Membrane:

    Article Title: Adipose stem cell-derived extracellular vesicles ameliorates corticosterone-induced apoptosis in the cortical neurons via inhibition of ER stress
    Article Snippet: Proteins were separated on 8%, 10% or 15% SDS-PAGE gradient gel and transferred onto PVDF transfer membrane (Thermo Fisher Scientific, Waltham, MA, USA). .. Then the membrane was incubated with rabbit anti-Caspase-3 (#9662, Cell Signaling Technology, Danvers, MA, USA), mouse anti-BAX (SC-20067, Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-Bcl2 (SC-7382, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-GRP78 (ab21685, Abcam, Cambridge, UK), rabbit anti-CHOP (MBS9606693, MyBioSource, San Diego, CA, USA), mouse anti-ATF4 (SC-390063, Santa Cruz Biotechnology, Dallas, TX, USA) and mouse anti-β-actin (A5316, Thermo Fisher Scientific, Waltham, MA, USA) at 4 °C overnight. .. Appropriate secondary antibodies conjugated to HRP were used (Thermo Fisher Scientific, Waltham, MA, USA) and the ECL reagents (Thermo Fisher Scientific, Waltham, MA, USA) were used for immunodetection.

    Article Title: Butylparaben Induces the Neuronal Death Through the ER Stress-Mediated Apoptosis of Primary Cortical Neurons.
    Article Snippet: Butylparaben is an organic compound that is used as an antimicrobial preservative in cosmetics and can cause neurotoxicity.. However, whether butylparaben induces neuronal death is unclear.. In this study, we report that butylparaben exposure induced neuronal apoptosis mediated by ER stress in primary cortical neurons.

    Incubation:

    Article Title: Adipose stem cell-derived extracellular vesicles ameliorates corticosterone-induced apoptosis in the cortical neurons via inhibition of ER stress
    Article Snippet: Proteins were separated on 8%, 10% or 15% SDS-PAGE gradient gel and transferred onto PVDF transfer membrane (Thermo Fisher Scientific, Waltham, MA, USA). .. Then the membrane was incubated with rabbit anti-Caspase-3 (#9662, Cell Signaling Technology, Danvers, MA, USA), mouse anti-BAX (SC-20067, Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-Bcl2 (SC-7382, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-GRP78 (ab21685, Abcam, Cambridge, UK), rabbit anti-CHOP (MBS9606693, MyBioSource, San Diego, CA, USA), mouse anti-ATF4 (SC-390063, Santa Cruz Biotechnology, Dallas, TX, USA) and mouse anti-β-actin (A5316, Thermo Fisher Scientific, Waltham, MA, USA) at 4 °C overnight. .. Appropriate secondary antibodies conjugated to HRP were used (Thermo Fisher Scientific, Waltham, MA, USA) and the ECL reagents (Thermo Fisher Scientific, Waltham, MA, USA) were used for immunodetection.

    Article Title: Butylparaben Induces the Neuronal Death Through the ER Stress-Mediated Apoptosis of Primary Cortical Neurons.
    Article Snippet: Butylparaben is an organic compound that is used as an antimicrobial preservative in cosmetics and can cause neurotoxicity.. However, whether butylparaben induces neuronal death is unclear.. In this study, we report that butylparaben exposure induced neuronal apoptosis mediated by ER stress in primary cortical neurons.

    Sonication:

    Article Title: MYC Drives mRNA Pseudouridylation to Mitigate Proliferation-Induced Cellular Stress during Cancer Development
    Article Snippet: .. Cross- linked chromatin was sheared through sonication (Fisher Scientific Model 150E) and immunoprecipitated using ChIP grade mouse anti-MYCN (B8.4.B, sc-53993, RRID:AB_831602), mouse anti-ATF4 (sc-390063, RRID:AB_2810998) or control mouse IgG (sc-2025, RRID:AB_737182) from Santa Cruz Biotechnology. ..

    Article Title: MYC Drives mRNA Pseudouridylation to Mitigate Proliferation-Induced Cellular Stress during Cancer Development
    Article Snippet: .. Cross-linked chromatin was sheared through sonication (Fisher Scientific, Model 150E) and immunoprecipitated using ChIP grade mouse anti-MYCN (B8.4.B, sc53993, RRID: AB_831602), mouse anti-ATF4 (sc390063, RRID: AB_2810998), or control mouse IgG (sc2025, RRID: AB_737182) from Santa Cruz Biotechnology. ..

    Immunoprecipitation:

    Article Title: MYC Drives mRNA Pseudouridylation to Mitigate Proliferation-Induced Cellular Stress during Cancer Development
    Article Snippet: .. Cross- linked chromatin was sheared through sonication (Fisher Scientific Model 150E) and immunoprecipitated using ChIP grade mouse anti-MYCN (B8.4.B, sc-53993, RRID:AB_831602), mouse anti-ATF4 (sc-390063, RRID:AB_2810998) or control mouse IgG (sc-2025, RRID:AB_737182) from Santa Cruz Biotechnology. ..

    Article Title: MYC Drives mRNA Pseudouridylation to Mitigate Proliferation-Induced Cellular Stress during Cancer Development
    Article Snippet: .. Cross-linked chromatin was sheared through sonication (Fisher Scientific, Model 150E) and immunoprecipitated using ChIP grade mouse anti-MYCN (B8.4.B, sc53993, RRID: AB_831602), mouse anti-ATF4 (sc390063, RRID: AB_2810998), or control mouse IgG (sc2025, RRID: AB_737182) from Santa Cruz Biotechnology. ..

    Article Title: Ssu72 phosphatase is essential for thermogenic adaptation by regulating cytosolic translation
    Article Snippet: Rabbit anti-eIF2! (Cell signaling Technology, Cat# 5324) is validated for Western blot analysis, immunoprecipitation, and immunohistochemical analysis; this antibody works for human, mouse, rat, and monkey (https://www.cellsignal.com/products/ primary-antibodies/eif2a-d7d3-xp-rabbit-mab/5324). .. Mouse anti-ATF4 (Santa Cruz Biotechnology, Cat# sc-390063) is validated for Western blot analysis, immunoprecipitation, immunofluorescence analysis, immunohistochemical analysis, and ELISA; this antibody works for human, mouse, and rat (https:// www.scbt.com/p/atf-4-antibody-b-3). .. Mouse anti-CHOP (Cell signaling Technology, Cat# 2895) is validated for Western blot analysis, immunoprecipitation, immunofluorescence analysis, flow cytometric analysis, and chromatin immunoprecipitation; this antibody works for human, mouse, and rat (https://www.cellsignal.com/products/primary-antibodies/chop-l63f7-mouse-mab/2895).

    Chromatin Immunoprecipitation:

    Article Title: MYC Drives mRNA Pseudouridylation to Mitigate Proliferation-Induced Cellular Stress during Cancer Development
    Article Snippet: .. Cross- linked chromatin was sheared through sonication (Fisher Scientific Model 150E) and immunoprecipitated using ChIP grade mouse anti-MYCN (B8.4.B, sc-53993, RRID:AB_831602), mouse anti-ATF4 (sc-390063, RRID:AB_2810998) or control mouse IgG (sc-2025, RRID:AB_737182) from Santa Cruz Biotechnology. ..

    Article Title: MYC Drives mRNA Pseudouridylation to Mitigate Proliferation-Induced Cellular Stress during Cancer Development
    Article Snippet: .. Cross-linked chromatin was sheared through sonication (Fisher Scientific, Model 150E) and immunoprecipitated using ChIP grade mouse anti-MYCN (B8.4.B, sc53993, RRID: AB_831602), mouse anti-ATF4 (sc390063, RRID: AB_2810998), or control mouse IgG (sc2025, RRID: AB_737182) from Santa Cruz Biotechnology. ..

    Control:

    Article Title: MYC Drives mRNA Pseudouridylation to Mitigate Proliferation-Induced Cellular Stress during Cancer Development
    Article Snippet: .. Cross- linked chromatin was sheared through sonication (Fisher Scientific Model 150E) and immunoprecipitated using ChIP grade mouse anti-MYCN (B8.4.B, sc-53993, RRID:AB_831602), mouse anti-ATF4 (sc-390063, RRID:AB_2810998) or control mouse IgG (sc-2025, RRID:AB_737182) from Santa Cruz Biotechnology. ..

    Article Title: MYC Drives mRNA Pseudouridylation to Mitigate Proliferation-Induced Cellular Stress during Cancer Development
    Article Snippet: .. Cross-linked chromatin was sheared through sonication (Fisher Scientific, Model 150E) and immunoprecipitated using ChIP grade mouse anti-MYCN (B8.4.B, sc53993, RRID: AB_831602), mouse anti-ATF4 (sc390063, RRID: AB_2810998), or control mouse IgG (sc2025, RRID: AB_737182) from Santa Cruz Biotechnology. ..

    Western Blot:

    Article Title: Ssu72 phosphatase is essential for thermogenic adaptation by regulating cytosolic translation
    Article Snippet: Rabbit anti-eIF2! (Cell signaling Technology, Cat# 5324) is validated for Western blot analysis, immunoprecipitation, and immunohistochemical analysis; this antibody works for human, mouse, rat, and monkey (https://www.cellsignal.com/products/ primary-antibodies/eif2a-d7d3-xp-rabbit-mab/5324). .. Mouse anti-ATF4 (Santa Cruz Biotechnology, Cat# sc-390063) is validated for Western blot analysis, immunoprecipitation, immunofluorescence analysis, immunohistochemical analysis, and ELISA; this antibody works for human, mouse, and rat (https:// www.scbt.com/p/atf-4-antibody-b-3). .. Mouse anti-CHOP (Cell signaling Technology, Cat# 2895) is validated for Western blot analysis, immunoprecipitation, immunofluorescence analysis, flow cytometric analysis, and chromatin immunoprecipitation; this antibody works for human, mouse, and rat (https://www.cellsignal.com/products/primary-antibodies/chop-l63f7-mouse-mab/2895).

    Analysis:

    Article Title: Ssu72 phosphatase is essential for thermogenic adaptation by regulating cytosolic translation
    Article Snippet: Rabbit anti-eIF2! (Cell signaling Technology, Cat# 5324) is validated for Western blot analysis, immunoprecipitation, and immunohistochemical analysis; this antibody works for human, mouse, rat, and monkey (https://www.cellsignal.com/products/ primary-antibodies/eif2a-d7d3-xp-rabbit-mab/5324). .. Mouse anti-ATF4 (Santa Cruz Biotechnology, Cat# sc-390063) is validated for Western blot analysis, immunoprecipitation, immunofluorescence analysis, immunohistochemical analysis, and ELISA; this antibody works for human, mouse, and rat (https:// www.scbt.com/p/atf-4-antibody-b-3). .. Mouse anti-CHOP (Cell signaling Technology, Cat# 2895) is validated for Western blot analysis, immunoprecipitation, immunofluorescence analysis, flow cytometric analysis, and chromatin immunoprecipitation; this antibody works for human, mouse, and rat (https://www.cellsignal.com/products/primary-antibodies/chop-l63f7-mouse-mab/2895).

    Immunofluorescence:

    Article Title: Ssu72 phosphatase is essential for thermogenic adaptation by regulating cytosolic translation
    Article Snippet: Rabbit anti-eIF2! (Cell signaling Technology, Cat# 5324) is validated for Western blot analysis, immunoprecipitation, and immunohistochemical analysis; this antibody works for human, mouse, rat, and monkey (https://www.cellsignal.com/products/ primary-antibodies/eif2a-d7d3-xp-rabbit-mab/5324). .. Mouse anti-ATF4 (Santa Cruz Biotechnology, Cat# sc-390063) is validated for Western blot analysis, immunoprecipitation, immunofluorescence analysis, immunohistochemical analysis, and ELISA; this antibody works for human, mouse, and rat (https:// www.scbt.com/p/atf-4-antibody-b-3). .. Mouse anti-CHOP (Cell signaling Technology, Cat# 2895) is validated for Western blot analysis, immunoprecipitation, immunofluorescence analysis, flow cytometric analysis, and chromatin immunoprecipitation; this antibody works for human, mouse, and rat (https://www.cellsignal.com/products/primary-antibodies/chop-l63f7-mouse-mab/2895).

    Immunohistochemical staining:

    Article Title: Ssu72 phosphatase is essential for thermogenic adaptation by regulating cytosolic translation
    Article Snippet: Rabbit anti-eIF2! (Cell signaling Technology, Cat# 5324) is validated for Western blot analysis, immunoprecipitation, and immunohistochemical analysis; this antibody works for human, mouse, rat, and monkey (https://www.cellsignal.com/products/ primary-antibodies/eif2a-d7d3-xp-rabbit-mab/5324). .. Mouse anti-ATF4 (Santa Cruz Biotechnology, Cat# sc-390063) is validated for Western blot analysis, immunoprecipitation, immunofluorescence analysis, immunohistochemical analysis, and ELISA; this antibody works for human, mouse, and rat (https:// www.scbt.com/p/atf-4-antibody-b-3). .. Mouse anti-CHOP (Cell signaling Technology, Cat# 2895) is validated for Western blot analysis, immunoprecipitation, immunofluorescence analysis, flow cytometric analysis, and chromatin immunoprecipitation; this antibody works for human, mouse, and rat (https://www.cellsignal.com/products/primary-antibodies/chop-l63f7-mouse-mab/2895).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Ssu72 phosphatase is essential for thermogenic adaptation by regulating cytosolic translation
    Article Snippet: Rabbit anti-eIF2! (Cell signaling Technology, Cat# 5324) is validated for Western blot analysis, immunoprecipitation, and immunohistochemical analysis; this antibody works for human, mouse, rat, and monkey (https://www.cellsignal.com/products/ primary-antibodies/eif2a-d7d3-xp-rabbit-mab/5324). .. Mouse anti-ATF4 (Santa Cruz Biotechnology, Cat# sc-390063) is validated for Western blot analysis, immunoprecipitation, immunofluorescence analysis, immunohistochemical analysis, and ELISA; this antibody works for human, mouse, and rat (https:// www.scbt.com/p/atf-4-antibody-b-3). .. Mouse anti-CHOP (Cell signaling Technology, Cat# 2895) is validated for Western blot analysis, immunoprecipitation, immunofluorescence analysis, flow cytometric analysis, and chromatin immunoprecipitation; this antibody works for human, mouse, and rat (https://www.cellsignal.com/products/primary-antibodies/chop-l63f7-mouse-mab/2895).



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    (A) Quantitative RT-PCR analysis of Xbp1 transcripts upon tunicamycin (Tuni) (1.2 μg/ml for 12 hr) or thapsigargin (Thap) (0.05 μM for 6 hr) treatment. Xbp1s: spliced Xbp1 transcript; Xbp1u: unspliced Xbp1 transcript; Ratio: ratio of spliced over unspliced Xbp1 transcript. (B) Representative images and quantification of nuclear <t>ATF4</t> positive MPP3 cells upon tunicamycin (1.2 μg/ml for 12 hr) or thapsigargin (0.05 μM for 6 hr) treatment. Scale bar: 10 μm. (C) Quantification of myeloid colony formation upon thapsigargin (Thap; 0.05 μM) and IXA4 (10 μM) treatment for 12 hr (n = 4). CFU, colony forming unit; MegE, megakaryocyte/erythrocyte; G (or) M, granulocyte or macrophage; GM; granulocyte/macrophage, GEMM, granulocyte/erythroid/macrophage/megakaryocyte. (D) Quantification of nuclear ATF4 positive MPP3 cells from vehicle versus tunicamycin treated control and Jak2 V617F mice. Data are means ± S.D., and statistical significance was assessed by a two-tailed unpaired Student’s t-test. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.
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    Image Search Results


    (A) Quantitative RT-PCR analysis of Xbp1 transcripts upon tunicamycin (Tuni) (1.2 μg/ml for 12 hr) or thapsigargin (Thap) (0.05 μM for 6 hr) treatment. Xbp1s: spliced Xbp1 transcript; Xbp1u: unspliced Xbp1 transcript; Ratio: ratio of spliced over unspliced Xbp1 transcript. (B) Representative images and quantification of nuclear ATF4 positive MPP3 cells upon tunicamycin (1.2 μg/ml for 12 hr) or thapsigargin (0.05 μM for 6 hr) treatment. Scale bar: 10 μm. (C) Quantification of myeloid colony formation upon thapsigargin (Thap; 0.05 μM) and IXA4 (10 μM) treatment for 12 hr (n = 4). CFU, colony forming unit; MegE, megakaryocyte/erythrocyte; G (or) M, granulocyte or macrophage; GM; granulocyte/macrophage, GEMM, granulocyte/erythroid/macrophage/megakaryocyte. (D) Quantification of nuclear ATF4 positive MPP3 cells from vehicle versus tunicamycin treated control and Jak2 V617F mice. Data are means ± S.D., and statistical significance was assessed by a two-tailed unpaired Student’s t-test. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.

    Journal: bioRxiv

    Article Title: Unfolded protein response signaling promotes myeloid cell production and cooperates with oncogenic mutation

    doi: 10.1101/2025.09.07.674755

    Figure Lengend Snippet: (A) Quantitative RT-PCR analysis of Xbp1 transcripts upon tunicamycin (Tuni) (1.2 μg/ml for 12 hr) or thapsigargin (Thap) (0.05 μM for 6 hr) treatment. Xbp1s: spliced Xbp1 transcript; Xbp1u: unspliced Xbp1 transcript; Ratio: ratio of spliced over unspliced Xbp1 transcript. (B) Representative images and quantification of nuclear ATF4 positive MPP3 cells upon tunicamycin (1.2 μg/ml for 12 hr) or thapsigargin (0.05 μM for 6 hr) treatment. Scale bar: 10 μm. (C) Quantification of myeloid colony formation upon thapsigargin (Thap; 0.05 μM) and IXA4 (10 μM) treatment for 12 hr (n = 4). CFU, colony forming unit; MegE, megakaryocyte/erythrocyte; G (or) M, granulocyte or macrophage; GM; granulocyte/macrophage, GEMM, granulocyte/erythroid/macrophage/megakaryocyte. (D) Quantification of nuclear ATF4 positive MPP3 cells from vehicle versus tunicamycin treated control and Jak2 V617F mice. Data are means ± S.D., and statistical significance was assessed by a two-tailed unpaired Student’s t-test. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.

    Article Snippet: Cells were then incubated overnight at 4 °C with a rabbit anti-mouse ATF4 (Cell Signaling, 11815S or Abcam, ab216839) primary antibody, washed 3 times with PBS, and incubated for 1 hour at RT with a goat anti-rabbit-A488 (Invitrogen, A32731) secondary antibody.

    Techniques: Quantitative RT-PCR, Control, Two Tailed Test